Evaluation of Ascochyta resistance in chickpea genotypes with quantitative polymerase chain reaction assay

In planta and soil quantification of Fusarium oxysporum f. sp. ciceris and evaluation of Fusarium wilt resistance in chickpea with a newly developed quantitative polymerase chain reaction assay.

Fusarium wilt of chickpea caused by Fusarium oxysporum f. sp. ciceris can be managed by risk assessment and use of resistant cultivars. A reliable method for the detection and quantification of F. oxysporum f. sp. ciceris in soil and chickpea tissues would contribute much to implementation of those disease management strategies. In this study, we developed a real-time quantitative polymerase ch...

Quantitative Real-time Polymerase Chain Reaction (qRT-PCR). Fatemeh Mehrpooyan*

Evaluation of in-house polymerase chain reaction assay sensitivity, can it be utilized in limited-resources settings?

  Background :Polymerase chain reaction (PCR) assay has widely used for the detection of tuberculosis (TB). This study tried to compare in-house PCR with some well-known commercial PCR kits for detection of TB agent.   Methods : Clinical samples obtained from 620 TB suspected patients were analyzed for the diagnosis of Mycobacterium tuberculosis complex (MTC) by in-house PCR. All samples were o...

Characterization of Iranian Isolates of Canine Parvovirus in Fecal Samples Using Polymerase Chain Reaction Assay

Despite the widespread prevalence of canine parvovirus disease (CPV) in Iranian dog population,molecular diagnosis of CPV variants, and investigation of the trends of its genetic changes is a new effort. Inthis study 50 samples from dogs suspicious of infection with clinical signs of diarrhea and vomiting, and 25samples from dogs suspected of infection with general symptoms su...

Polymerase chain reaction assay targeting nox gene for rapid identification of Brachyspira canis in dogs

Genus Brachyspira,as Gram negative anaerobic bacteria, colonize in dogs intestine. The aim of the current study was to determine the prevalence of Brachyspira spp. for the first time in Iran and rapid identification of Brachyspira spp. in dogs by a new designment of a species-specific primer set for B. canis. One hundred fifty-one fecal samples were obtained ...